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KMID : 1159320180200030249
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Korean Journal of Vision Science
2018 Volume.20 No. 3 p.249 ~ p.258
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Antimicrobial Susceptibility test and DNA gyrase analysis of Coagulase-negative Staphylococci isolated
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Na Hun-Taek
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Abstract
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Purpose : We investigated the susceptibility of quinolone series and vancomycin, erythromycin antibiotics for isolates of Coagulase-negative Staphylococci and analyzed the DNA gyrase gene.
Methods : Antibiotic susceptibility ofloxacin, ciprofloxacin, levofloxacin, vancomycin, and erythromycin were studied in six strains of coagulase-negative Staphylococci detected in corneas of suspected bacterial keratitis. Antibiotic susceptibility ofloxacin, ciprofloxacin, levofloxacin, vancomycin, and erythromycin were studied in six strains of coagulase-negative Staphylococci detected in corneas of suspected bacterial keratitis. and the genomic DNA of each strain was extracted. After amplifying PCR, DNA of gyraseA gene was analyzed.
Results : In the antibiotic susceptibility test, ofloxacin, ciprofloxacin, levofloxacin and vancomycin susceptibility, and erythromycin resistance were observed in strains of S. epidermidis (SE-2, SE-3), S. hominis (SH-1) and S. saprophyticus (SS-1), showing that all of the other strains were susceptible. In the gyraseA gene analysis, amino acid double mutation was observed from S. epidermidis (SE-1) strain as Met75→Thr and Met93→Val, and many silent mutations were observed in strains of S. warneri (SW-1) and S. saprophyticus (SS-1).
Conclusions : The isolates of Coagulase-negative Staphylococci showed resistance to erythromycin antibiotics, and amino acid double mutation and silent mutation were observed from gyraseA gene analysis. Therefore, although it is not resistance now, potential mutations would be thought that can cause resistance at any time.
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KEYWORD
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Coagulase-negative Staphylococci , Antibiotic , gyraseA , Mutation
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